What is the safety and efficacy of platelet-rich plasma (PRP) in adults pertaining to wound healing and aesthetic rejuvenation? As use of PRP expands, a growing number of physicians and patients seek to answer this question. Barring large-scale clinical trials, a systematic literature review offers the best chance of answering this question. Per the Cochrane collaboration, a systematic review evaluates and compiles the findings of multiple clinical trials and provides best evidence to address the chosen research question. Conclusions can then be made and future recommendations suggested.
A comprehensive search of the current literature was undertaken using three databases—Cochrane Library, Medline via the Pubmed interface, and Google scholar—for an extensive review. The search parameters were limited to all English Language articles from 2006 to 2016, excluding animal studies, in vitro studies, and articles with no measureable endpoint.
The search strategy was designed to select all relevant articles via Mesh terms combined with key text terms to address our research question. Different constructs of search terms were formed by the implementation of truncation of the following terms: PRP wound healing, PRP facial rejuvenation, PRP, platelet-rich plasma, platelet preparations, PRFM platelet-rich fibrin matrix, growth factors, platelet therapy, platelet facial platelet wound healing, platelet plastic surgery AND facial rejuvenation or aesthetic rejuvenation or wound healing
Initial search yielded 552 publications from Pubmed, nine from Cochrane Library, and 1,470 from Google Scholar. The search term was refined further to identify relevant articles, adhering to the steps of Prisma 2009. A review of the title and abstract of these papers was undertaken to determine their relevance to our research question, and this then yielded 23 potentially relevant papers. Scanning the references of these papers yielded further studies. Next, an in-depth analysis of these papers was carried out to ascertain the level of evidence, quality, and whether they met all of our inclusion and exclusion criteria. Six systematic reviews and one critical review were eventually extracted.
1. Search ((“Platelet-Rich Plasma”[Mesh] AND “last 10 years”[PDat] AND Humans[Mesh])) AND (facial rejuvenation or wound healing) – 552 results on Pubmed
2. Search (safety or efficacy ) AND “Platelet-Rich Plasma”[Mesh] Sort by: Relevance Filters: published in the last 10 years; Humans – 325 results on Pubmed
3. “Platelet-Rich Plasma”[Mesh] AND “last 10 years”[PDat] AND Humans[Mesh])) AND (facial rejuvenation or wound healing)) AND “last 10 years”[PDat] AND Humans[Mesh])) AND (systematic review or critical review) – 161 results on Pubmed
4. Search ((((safety or efficacy ) AND “Platelet-Rich Plasma”[Mesh]) AND “last 10 years”[PDat] AND Humans[Mesh])) AND systematic review Sort by: Relevance Filters: published in the last 10 years; Humans – 102 results on Pubmed
5. Search (((((((safety or efficacy ) AND “Platelet-Rich Plasma”[Mesh]) AND “last 10 years”[PDat] AND Humans[Mesh])) AND systematic review) AND “last 10 years”[PDat] AND Humans[Mesh])) AND WOUND HEALING Sort by: Relevance Filters: published in the last 10 years; Humans – 39 results on Pubmed
6. Search (((((((safety or efficacy ) AND “Platelet-Rich Plasma”[Mesh]) AND “last 10 years”[PDat] AND Humans[Mesh])) AND systematic review) AND “last 10 years”[PDat] AND Humans[Mesh])) AND AESTHETIC REJUVENATION Sort by: Relevance Filters: published in the last 10 years; Humans – 0 results on Pubmed
7. ‘Platelet rich plasma’ – 9 results on Cochrane
8. ‘platelet rich plasma and wound healing and facial rejuvenation’ – 1470 results on google scholar
9. ‘platelet rich plasma and (wound healing or aesthetic rejuvenation’ – 612 results on google scholar
10. ‘platelet rich plasma and wound healing and aesthetic rejuvenation’ – 589 results on google scholar
Steps in a systematic review, as per Cochrane:
1. Identify your research question.
2. Search for studies.
3. Define inclusion and exclusion criteria.
4. Extract studies that fulfill the above criteria.
5. Perform data analysis of selected studies.
6. Evaluate the degree of bias of the above.
7. Present findings and assess quality and level of evidence.
What is the safety and efficacy of platelet-rich plasma in adults pertaining to wound healing and aesthetic rejuvenation?
Target population – Adults
Intervention - Aesthetic Rejuvenation and Wound Healing with platelet-rich-plasma
Outcome(s) – Safety, Efficacy
Efficacy. Of the six systematic reviews and one critical review, five evaluated various types of wound healing from post-surgical ulcers, sports related, and two evaluated aesthetic rejuvenation. Studies varied in design, size, and power, hence this lack of uniformity results in complexity in comparison, and poor validity and reproducibility of findings. Reports varied in outcome measures.
In wound healing, common parameters used were complete wound healing, wound area reduction, wound depth, healing rate, time to heal, complications, example wound infection, quality of life, and pain reduction. As wounds cover a wide spectrum, from medical to post surgical, and consist of a wide degree of variation, the studies unfortunately did not indicate a detailed description of the type of wounds investigated to correct for inter wound variability.
In aesthetic rejuvenation, common parameters used were subjective doctor and patient evaluation, photo reviews, and objective skin biopsy, trichoscan hair measurements, etc. Noting the above variation in outcome measures, findings tend to lack statistical power. Several studies lacked controls and split-face comparisons. Studies were often small scale, with few subjects.
Adults > 19 years old
Skin rejuvenation or Wound healing
Timeframe for 2006 to 2016
English language publications
Highest quality papers with level 1 evidence
In vitro studies
Articles with no measurable endpoint
RCT: randomized controlled trials
PRP: Platelet rich plasma
There is a high likelihood of publication bias favoring positive results with the use of PRP in both cases, which demonstrated positive effects on cellular changes, wound healing and aesthetic rejuvenation. Many of the studies demonstrated results that were difficult to quantify objectively. PRP treatment protocol lacked standardization for extraction, injection, dosing, and volume. Lack of studies evaluating different forms of PRP and variation in the PRP collection process, which leads to difficulty in comparing studies.
Safety. On the aspect of safety, PRP is superior to filler agents, with self-limiting minor side effects of swelling, erythema and bruising as per QMUL module 8 coursework. The above studies have not reported any long-term or permanent side effects.
Clinical application. With reference to the research question, our findings show that although results are encouraging, high level evidence and high quality RCTs are still lacking. That said, the evidence for wound healing appears to be more substantial than for aesthetic rejuvenation as seen by the quality, design, and number of RCTs.
There is still a wide variation in the types and uses of platelet preparation, with no universal standard. Combination of PRP with other modalities is a big scope for future evaluation, specifically the choice, dosing, sequencing, interval, and frequency.
Large robust RCTs are needed, however a suitable split-face intervention as a control to PRP has yet to be established. Another challenge would be the fact that pharmaceutical companies would hesitate to fund a trial with no intellectual property rights.
Martinez-Zapata MJ, Marti-Carvajal A, Sola I, et al. Efficacy and safety of the use of autologous plasma rich in platelets for tissue regeneration: a systematic review. Transfusion. 2009;49(1):44-56.
Villela DL, Santos VL. Evidence on the use of platelet-rich plasma for diabetic ulcer: a systematic review. Growth Factors. 2010;28(2):111-6.
Carter MJ, Fylling CP, Parnell LKS. “Use of platelet rich plasma gel on wound healing: a systematic review and meta-analysis.” ePlasty: Open Access Journal of Plastic Surgery 11 (2011).
Taylor DW et al. “A systematic review of the use of platelet-rich plasma in sports medicine as a new treatment for tendon and ligament injuries.” Clinical journal of sport medicine 21.4 (2011): 344-352.
Leo MS et al. “Systematic review of the use of platelet‐rich plasma in aesthetic dermatology.” Journal of cosmetic dermatology 14.4 (2015): 315-323.
Sclafani AP, Azzi J. “Platelet preparations for use in facial rejuvenation and wound healing: a critical review of current literature.” Aesthetic plastic surgery 39.4 (2015): 495-505.
Wang L, Gu Z, Gao C. “Platelet-rich plasma for treating acute wounds: a meta-analysis.” Chinese Medical Association PH (2014): 2169-2174.